Abstract :Plants have always been used by humans to find cures for its diseases. After a prosperous period for synthetic molecules, the craze for natural plant substances is now a fact. The expanding market for medicinal plants, because of globalization, requires technical botanical identifications, standardized, to allow a secure commerce. The aim of our work is to establish a pharmacognostical standard methodology, through various examples, leading to useful standards trade of medicinal plants. This study, divided into three parts, include macroscopic and microscopic analysis of Origanum compactum, Opuntia ficus indica, Vitex agnus castus and Stephania tetrandra. Another part of this work concerns the screening phytochemicals such as TLC for Viburnum, Hieracium pilosella and Secale, HPLC chromatographic profiles for Vitex agnus castus and Stephania tetrandra and UV dosage on Hieracium pilosella, which allowed us to highlight the main phytochemical markers for each plant, for example. These results provide on the one hand, qualitative characterization by TLC and also quantitatively by HPLC and UV dosage. The last part of our work concerns the evaluation of physico-chemical characteristics of Hieracium pilosella, Piper nigrum and Piper longum. This work shows the usefulness of such analysis to create a standardized method for botanical and chemical identification, always necessary before using medicinal plants.